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Following incubation, caspase 3 activity was measured using the fluorogenic substrate AC-DEVD-AMC as described before ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells

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Types Of Anesthesia While there are a few different forms of anesthesia, two main types are commonly used during breast augmentation: General Anesthesia: What It Is: General anesthesia involves medications that make you fully unconscious