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Samples were transferred to Miro5 buffer (pH 7.1, 110 mM sucrose, 60 mM potassium lactobionate, 20 mM taurine, 10 mM monobasic potassium phosphate, 3 mM magnesium chloride, 20 mM HEPES, 0.5 mM EGTA, 1 mg/mL BSA) and washed for 3 10 min on a rocker at 4 C