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Kinetic BRET measurements were taken at 37C using a Spark plate reader (Tecan) with 460/40 nm and 535/25 nm filters for RLuc8 and Venus emissions, respectively
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The increased -MSH activates the melanocortin four receptor (MC4R) (140), which in turn mobilizes the sympathetic nervous system, inhibits insulin secretion, enhances hepatic insulin sensitivity (141), and suppresses appetite by modulating NPY/AgRP neurons that promote insulin (142)
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