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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)
Thus, the ability of TAT-DAT NT to disrupt the D2R-DAT interaction should not be altered, which was confirmed by our results in Fig
McCarty, J
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