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injection of 100 nmol kg 1 MK-801 ( n = 8 mice), 100 nmol kg 1 GLP-1 ( n = 8 mice), 100 nmol kg 1 , GLP-1MK-801 ( n = 8 mice), or vehicle (isotonic saline, n = 8 mice) in DIO mice

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Key Takeaway Chromatography ensures separation and quantification precision, complementing spectroscopys structural depth

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Data acquisition was done using SerialEM software at 500 nm defocus 44

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