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After 3 h of OGD treatment in HUVECs, WB results showed that the expression of Ki-67 and Lamin B decreased, while the expression of P53, P21, P16, and -H2AX significantly increased (Figure 3A)
14 Current assays have limited capacity to distinguish among distinct resistance pathways, such as target mutations, bypass pathway activation, or phenotypic transitions
Many protocols use them together for enhanced results
MTT (5 mg) was placed in a 5 mL EP tube, and PBS buffer (1 mL) added to dissolve the MTT
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