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The BSA encapsulation efficiency was calculated using the equation below: BSA encapsulation efficiency = (initial BSA concentration BSA concentration in supernatant)/(initial BSA concentration) * 100 Sample calculation: Initial BSA concentration added: 2 mg/mL BSA concentration in the supernatant: 0.49 mg/mL Encapsulation efficiency= (2 0.49)/(2) * 100 = 75.5% Protein release measurements We resuspended nanoparticles in phosphate buffered saline (12), and placed samples at different temperatures: 4C and 37C
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