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Yiannikourides A, Latunde-Dada GO (2019) A short review of iron metabolism and pathophysiology of iron disorders
ShivakotiRChristianPYangWTGupteNMwelaseNKanyamaCet al
General advice is not good enough when you are working with compounds that affect gene expression at the cellular level
RNA was isolated from brain and islets using RNeasy micro kit (Qiagen), cDNA was synthesized from equal amounts of RNA using SuperScript III First-Strand Synthesis kit (Invitrogen) and gene expression was quantified using PowerUp Sybr Green Reagents (Invitrogen) according to the manufacturers instructions
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