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Helmi, Fakhrudin, N., Nurrochmad, A., Sudarmanto, B
SDS-PAGE and Western blotting Samples were loaded onto 10% SDS-polyacrylamide gels then transferred to 0.45-m PVDF membranes and blocked with 5% skimmed milk [or 5% bovine serum albumin (BSA) for anti-phospho antibodies] in TBS buffer supplemented with 0.1% Tween 20 (TBS-T)
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