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Sourcing quality is the primary risk
If you are getting fewer doses than expected, you may be drawing more than your target volume
Also, proteins and peptides can be metabolized in the cecum and colon [189], as protein fermentation lead to branched SCFAs production [188]
Following the indicated experimental design, 5 10 4 FACS-sorted ILC2s were plated on a Seahorse XFp PDL-coated cell culture miniplate in triplicates in FBS/Phenol red free Seahorse media supplemented with 1 mM pyruvate, 2 mM glutamine, and 10 mM glucose
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