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Cells were maintained in DMEM (HCT116, SW480, U2OS), MEM- with 1 ng ml 1 fibroblast growth factor 2 (Peprotech, hMSC) or DMEM with 1% non-essential amino acid (Gibco) and 50 g ml 1 uridine (Sigma-Aldrich) supplementation (143B), with 10% dialysed foetal bovine serum (dFBS), 50 U ml 1 penicillin and streptomycin (ThermoFisher, 15070063) and 1 mM sodium pyruvate (ThermoFisher, 11360070)
Both glutathione and NAC support skin health, but they work differently
For example: Remove inflammatory foods and obvious food triggers
There are millions of processes taking place in our bodies in combination with environmental factors that can lead to oxidative stress affecting our cells, immune system, and important detoxification processes
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