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used subcellular fractionation and imaging to confirm plasma membrane localisation and to demonstrate that myristoylation and flavin adenine dinucleotide (FAD)dependent oxidoreductase activity are both required for ferroptosis suppression, with nonmyristoylated or catalyticdead FSP1 variants failing to rescue ferroptosis despite comparable expression

Its small size and copper-binding affinity underpin its bioactivity, distinguishing it from larger proteins or synthetic peptides