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Sep 2012;24(9):791-801
The Most Common Problem: Powder That Will Not Dissolve A peptide that does not dissolve after standard reconstitution is not necessarily defective
To assess the LMW persulfides, 100 L samples were precipitated with 1 mL of absolute MeOH at 40 C for 2 h, centrifuged (16,000 g at 0 C), and obtained supernatants were evaporated and crude residues were resuspended and sonicated in 100 L 50 mM HEPES buffer (pH 7.4)
The immunoreactive bands were detected using the Odyssey Infrared Imaging System (LI-COR Biosciences)
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