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After the washing step the R6G dye of the internalized non-targeted or targeted nanocarriers in living hECs was imaged using the 543 nm laser line on a Leica TCS SP5 confocal laser scanning microscope equipped with a sample holder kept at 37 C
Furthermore, these theories do not provide an explanation to the evolutionary-conserved roles of sleep
The book is highly recommended for researchers in the field of sleep neurobiology, although some minor weaknesses such as content overlap and the absence of certain topics are noted
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