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Add 50 L of HRP-Streptavidin working solution to each well, cover with a plate sealer, and incubate at 37 C for 30 min
People with sensitive skin often experience discomfort or irritation more quickly and intensely than those with normal skin
Protein intake is particularly important, as the thermic effect of protein (the energy required to digest and process it) is higher than that of carbohydrates or fats, contributing to heat production
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