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Follow the instructions in the H-FABP ELISA kit (JM-04323H2, Jingmei, Jiangsu, China) manual to determine H-FABP levels in each groups serum using a multifunctional enzyme analyzer (Molecular Devices, Shanghai, China)

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Peptide Sciences Applications in Research As a molecular probe to study NNMT function, regulation, and downstream metabolic pathways To investigate metabolic flux , methylation dynamics, NAD salvage, and related bioenergetic phenomena In cellular assays , to test effects of NNMT inhibition on gene expression, metabolite profiling, or metabolic phenotypes In animal / ex vivo experiments for mechanistic elucidation of metabolic regulation (e.g