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Peptides were dissolved in running buffer and the stock concentrations determined using the NanoDrop 2000 spectrophotometer (Thermo Fischer Scientific) at a wavelength of 280 nm
They slow gastric emptying and reduce appetite and food intake
Deviating from this established protocol could mean losing the benefits that years of clinical data support
54 Therefore, although we found that cinchonine lowered blood glucose and ameliorated NASH by activating GLP-1R, it cannot be excluded that it has other mechanisms to enhance or attenuate these effects
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