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rhamnosus GG were cultured in TSB supplemented with 100 M nitrite solution (Sigma-Aldrich, 72586, Ireland Ltd.) until they reached 10 8 CFU/mL
A 200mcg dose from a vial reconstituted with 3mL requires 0.12mL injection volume vs 0.08mL from a 2mL reconstitution the larger volume takes longer to inject and creates more tissue displacement
Benefits #1st micellar water created, original formula
Table 1 Multi-weaponry approach involving glutathione (GSH) enhancers, nuclear factor erythroid 2 p45related factor 2 (Nrf2) activators, toll-like receptor (TLR) inhibitors/immunomodulators, C-reactive protein (CRP) level reduction, natural and immune immunoglobulin M (IgM) enhancement and immune cell function recovery against SARS-CoV-2 infection and COVID-19 disease
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