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Macrophage polarization and M1/M2 imbalance are pathological features of many inflammatory diseases (Shapouri-Moghaddam et al., 2018)
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One outlier was removed from the vehicle, MK-801 and GLP-1MK-801 groups due to deviation related to tissue processing
After that 20 l of 1250 U/ml mushroom tyrosinase solution was added into each well to a final volume 250 l and incubated at 25C for another 5 minutes
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