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Lastly, it is important to exercise caution when directly comparing detailed results between the main and exploratory cohorts, as well as between the 1st- and 2nd-year follow-up periods, because sample sizes differed due to varying inclusion/exclusion criteria and data availability
The amplification cycle consisted of an initial denaturation at 98 C for 30 sec, followed by 26 cycles of 10 sec at 98 C, 20 sec at 51 C, and 1 min at 72 C
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Marchi G, Busti F, Zidanes AL, et al
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