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3.6 Cystine stimulates the expression of oxidative stress genes following gentamicin treatment To further confirm that the addition of cystine induced an imbalance in intracellular redox levels, thereby causing cellular oxidative stress, we examined the expression of oxidative stress-related genes, including OxyR , and the SOS response genes lexA , recA , and umuD ( lexA , recA , umuD , and OxyR expression following the addition of cystine alone (Figure 7A), which aligned with our expectations