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Tumours and tissues were snap-frozen on dry ice and stored at 80 C or placed in 10% neutral buffered formalin (Fisher Scientific, 22-110-761) for 24 h and then stored in 100% methanol (Fisher Scientific, A412P-4)

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In prior years, if a firm was missing a single premium value, this value was imputed using a hot deck approach in which a single premium was selected among firms with similar demographic characteristics