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Freezing a mixed multi-use vial can create solution changes and freeze-thaw stress that are not worth accepting unless a protocol specifically requires aliquoted frozen storage

Abbreviations A: Amyloid- AD: Alzheimers disease BBB: Blood-brain barrier BDNF: Brain-derived neurotrophic factor CNS: Central nervous system dAGEs: Dietary advanced glycation products DCI: Diabetic cognitive impairment ENS: Enteric nervous system GABA: Gamma-aminobutyric acid GDNF: Glial cell line-derived neurotrophic factor HFD: High-fat diet HPA: Hypothalamic-pituitary-adrenal IL-1: Interleukin-1 beta INSR: Insulin receptor MGBA: Microbiome-gut-brain axis MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine NDDs: Neurodegenerative diseases PD: Parkinsons disease PRISMA: Preferred reporting items for systematic reviews and meta-analyses ROS: Reactive oxygen species STZ: Streptozotocin SCFAs: Short-chain fatty acids SNpc: Substantia nigra pars compacta SYRCLE: Systematic review centre for laboratory animal experimentation TH: Tyrosine hydroxylase TNF-: Tumor necrosis factor-alpha References Makdissi S, Parsons BD, Di Cara F

20 With a prolonged clinical course, sequential reactivation of various human herpesviruses (particularly type 6 and type 7) and, less frequently, Epstein-Barr virus and cytomegalovirus infections, may be seen