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The fact that the number of oocytes obtained in the summer season did not differ from that collected during the rest of the year in terms of nuclear maturation and the cleavage rate could indicate that immature oocytes can be fertilized and undergo cleavage without developing to the blastocyst stage 41
The elements of aseptic technique are an aseptic work area, good personal hygiene, sterile (or sterile filtered) reagents and media, and aseptic handling
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