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AGEs and other products of glycation, whether formed endogenously or derived exogenously, may have similar biological effects and are mainly known for their harmful impact, therefore, the term glycotoxins is used to emphasize the toxicity of certain of them

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The membrane was washed three times in Tris-buffered saline with Tween 20 (TBS-T) for 10 min, and treated with HRP-conjugated anti-rabbit or anti-mouse IgG (GE Healthcare) for 1 h