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Injected oocytes were stored at 18 C in ND96 storage buffer (96 mM NaCl, 2 mM KCl, 1 mM MgCl 2 , 1.8 mM CaCl 2 , 5 mM hemisodium-HEPES, pH 7.4 supplemented with 50 g/ml gentamycin, 2.5 mM sodium pyruvate, 50 g/mL tetracycline and 0.5 mM theophylline) for three days to allow the expression of hOCTN2 on the oocyte plasma membrane
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When its oxidized, glutathione is inactive and cannot fulfill its role as an antioxidant
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