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Plant-derived inhibitors obtusilactone A and sesamin effectively block LONP1 proteolytic function by forming hydrogen-bond networks with serine 855 and lysine 898 residues in its active site, thereby inducing apoptosis in lung cancer cells [141]
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The samples were precipitated at -20 C for 1 h, centrifuged at 12,000 rpm for 10 min at 4 C, and the supernatant was collected for LC-MS analysis
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