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Centrifuge at 1,500g for 10 minutes at 28C
Glia serve as a major supplier of cysteine for neuronal glutathione synthesis, and 50-60% of glutamate neurotransmitter is derived from the glutamine-glutamate shuttle between neurons and glia, with substantially smaller amounts of glutamate transmitter derived from glycolysis (12-14)
Inadequate protein during a caloric deficit leads to muscle loss rather than fat loss, defeats the purpose of the protocol, and creates the metabolic slowdown that causes weight loss plateaus
Research shows that MK677 increases the secretion of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) without affecting cortisol levels
