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CRISPR/Cas9-mediated knockout and knockin was performed using the Neon Transfection System (Thermo Fisher Scientific): Synthetic CRISPR guide RNAs, ssODN Donors and recombinant Cas9 (IDT) were electroporated according to the manufacturers protocol and recommendations (1005 V, 35 ms, 2 pulses)

This cautious approach prevents unnecessary discomfort from applying potentially irritating products directly to delicate periorbital region, following peptide safety protocols minimizing risk

Pack Size & Treatment Yield

Years ago, we made the surprising discovery that prostaglandins (a type of fatty acid), play an important role in male brain development