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The Role of Pancreatic Preproglucagon in Glucose Homeostasis in Mice
~100 worms were collected in 500 l PBST (PBS + 0.1% Tween-20), washed once with PBS, and fixed in 4% formaldehyde fixative (800 l water, 100 l 10 PBS, and 100 l formaldehyde) for 30 min
Where the complex does partially survive, on-column dissociation and re-equilibration during the run produce peak splitting, shoulders and pronounced tailing, and these are chemistry rather than a bad column
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