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Following this, the protein lysates were denatured in a metal bath at 95 C and subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) for separation

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For one thing, the ageing process in humans is much slower than in rats, so the seven-week supplementation period used in the rat studies would equate to around five years of supplementation in humans

This article covers the exact reconstitution protocol used in controlled laboratory settings, the sterile technique required to prevent contamination, and the concentration calculations that ensure dosing precision across multi-week studies