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Size does not determine alone the fate of nanocarriers, the same permeability values were measured for polystyrene nanoparticles with 100, 200 or 400 nm diameter across a microfluidic BBB model [59]
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The homogenization of the frozen samples, protein measurements, electrophoresis, primary and secondary antibody incubation and quantification were performed following our previously published protocols (Gergs et al
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