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Spatial analysis techniques further allow investigators to examine the distribution of these microorganisms across different intestinal regions and assess their interactions with host metabolic pathways

Pathway analysis and tissue expression To identify enriched biological pathways associated with altered tear fluid proteins in amyloid PET-positive and negative participants, we conducted Gene Set Enrichment Analysis (GSEA) in R

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Liquid chromatographymass spectrometry All peptide samples were separated and analyzed on an online liquid chromatographytandem mass spectrometry (LCMS/MS) system, consisting of a Vanquish Neo ultrahigh-performance LC (Thermo Fisher Scientific) coupled to an Orbitrap Exploris 480 (Thermo Fisher Scientific)