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Conversely, the endosomal TLRs, consisting of TLR3, TLR7, TLR8, TLR9, and TLR13 (exclusive to mice), specialize in detecting nucleic acids

This makes it particularly useful in acute situations

Antibodies employed in this study were specific for the following molecules: CD3 (clone OKT3 mouse mAb), CD28 (clone 28.2, BD Pharmingen), isotype control antibodies IgG1 and IgG2a (mouse mAb, BD Biosciences), cysteine sulfenic acid (rabbit polyclonal antibody, Merck), Prx1 (peroxiredoxin1, rabbit polyclonal antibody, Invitrogen), Trx (thioredoxin, Clone 2G11, mouse mAb, BD Bioscience), phospho-STAT3 (pSTAT3, #9131, Cell Signaling Technology), STAT3 (#9139, Cell Signaling Technology), and GAPDH (clone 6C5, mouse mAb, Invitrogen)