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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

Laboratory Certification : Every batch of BPC-157 is manufactured in a GMP-certified facility, ensuring compliance with Good Manufacturing Practices, which guarantees consistency, quality, and safety

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First isolated and characterized in the early 1990s, BPC-157 peptide has become one of the most extensively studied peptides in preclinical research, with hundreds of published studies exploring its properties in laboratory and animal models