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Here are the most common ones: Injecting water directly onto the lyophilizate , a strong jet can denature the peptide
Substrate: 0.9 M Testosterone Incubation: 30 min at 37C Control Inhibitor: Dutasteride Criteria For Significance: 50% of max stimulation or inhibition Test Concentration / Dose: Suggested concentration for initial testing is 10 uM Test Sample Requirements: Minimum for 1) Screen: 25 l of 10 mM stock -OR- 1 mg (pre-weighed)
Farkas, O
The active component turned out to be the tripeptide glycine-histidine-lysine, which binds copper to form GHK-Cu
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