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The glycan chain at Asn297 was trimmed using the endoglycosidase Endo S2 and then azide groups were introduced using a mutant galactosyl transferase GalT(Y289L) and N -azidoacetylgalactosamine (GalNAz)
Systemic Inflammation: By preventing the accumulation of toxic lipid intermediates in the cellular cytoplasm, L-carnitine reduces the generation of reactive oxygen species (ROS), helping to lower systemic inflammatory markers like C-reactive protein (CRP) and stabilize mast cell reactivity
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