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If a lump persists beyond 2 weeks, hardens significantly, or becomes increasingly painful, contact a healthcare provider

The column was washed with 20 ml equilibration solution, and elution was performed with a linear salt gradient from 37.5 to 162.5 mmol/kg KCI (20 mmol/kg citric acid, 45 % (w/w) ethanol, pH 3.5) followed by 20 ml of isocratic elution at 250 mmol/kg KCI (20 mmol/kg citric acid, 45 % (w/w) ethanol, pH 3.5)