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All resulting slides were digitally scanned using the Hamamatsu Nano Zoomer S60 and provided to the Study Pathologist, Sureshkumar Muthupalani, BVSc, MVSc, PhD, DACVP, to review staining success and quality

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Considering the fact that Baf-A1 suppresses fusion of autophagosomes with the lysosomes 39 , our finding that DFO protected against ferroptosis suggests that lysosomal iron pool is involved in GSH depletion-induced cell death 48