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2003 May;101(5 Pt 1):9951007
Treatment with NAC restored the sizes of ACTL6A-KD PDOs that had been decreased, and enabled organoids regained a cystic structure (Fig
Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution
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