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The work moves through a clear sequence of stages, and the output of each stage is reviewed before the next begins: Secondary research forms the foundation of the study and shapes the questions used later in primary interviews

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Real-time quantitative polymerase chain reaction Total RNA was extracted from cells using the Trizol reagent (Takara Bio Inc, Japan) according to the manufacturers instructions

doi:10.4093/dmj.2022.0221 20