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Gentle mixing, swirling rather than shaking, is typically recommended, since vigorous agitation can denature the peptide's tertiary structure and reduce activity in downstream assays

B 117 (39), 1150211508

* Correspondence: Vishwanath Venketaraman, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers