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Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles
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pmc.ncbi.nlm.nih.gov/articles/PMC12313605/?utm_source=chatgpt.com
In particular, the loss of binding of GSH may debilitate the stability of 14S pentamers, resulting in their failure to assemble into mature virus
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