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CCM measures parameters including corneal nerve fiber density (CNFD), corneal nerve branch density (CNBD) and corneal nerve fiber length (CNFL), all of which are significantly reduced in patients with subclinical and clinical DPN compared with controls
Real-Time RTPCR Total RNA was extracted from BAT using the RNeasy Mini Kit (QIAGEN, Hilden, Germany) and stored at 80 C
All cofactors for enzymes that peak between 6 a.m
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