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Western blot analysis and Coomassie Brilliant Blue (CBB) staining Samples were boiled in sample loading buffer at 100 C for 10 min, separated by SDS-PAGE, and transferred onto NC membranes for blotting

This is where you'll see the earliest changes and confirm copper levels remain in range

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Although these therapies reduced CRP in the majority of the interventions (80 %, 4/5) (Table 2), both UV radiation and psoralens generate free radicals, potentially inducing oxidative stress [67]

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