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The membrane was incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified polyclonal antibody (Catalog # A02059-1) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT
Serious but rare risks include pancreatitis, gallbladder disease, acute kidney injury (usually in the context of severe dehydration from gastrointestinal side effects), and hypersensitivity reactions including anaphylaxis and angioedema
Journal of nutritional science and vitaminology
The population was primarily between the ages of 45-64 (51.9%), female (55.5%), white (71.8%), and not Hispanic or Latino (82.9%)
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