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In the diabetes study, no patients discontinued CagriSema or cagrilintide due to adverse effects, whereas one patient discontinued semaglutide due to adverse effects [10]
The protein samples (12 L) and Spectra Multicolor Broad Range Protein Ladder (7.5 L) with 10260 kDa range (Thermo Fisher Scientific) were loaded onto the gel, and electrophoresis was run at 200 V for 22 min in 1 Bolt MES SDS Running Buffer (Invitrogen)
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As control of HA2X formation, 79 DIV cultured neurons were treated with doxorubicine (0.5 M) for 24 h, fixed in 4% PFA in PBS and analyzed in immunofluorescence as described above
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