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44 Total RNA was reverse transcribed into first-strand complementary DNA and RT-PCR was performed using a LightCycler Real-Time PCR system (Roche, Switzerland) as previously described

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Epidemiological studies showed that genes contribute to the vulnerability of multiple CNS disorders, such as major depressive disorders (MDD), schizophrenia and substance use disorders with heritability respectively of 3142%, 6480%, and 2379% [10,11,12,13]