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The mitochondrial pellet was resuspended in MSHE buffer with BSA and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific)

Based on a 1999 review in Med Sci Sports Exerc., by Powers, Ji & Leeuwenburgh Exercise training-induced alterations in skeletal muscle antioxidant capacity: a brief review Introduction The Invisible Battle in Every Workout Every time you lace up your shoes and push your body through a run, a ride, or a set of squats, something invisible happens deep inside your muscles