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A typical B12 shot contains 1000mcg of B12, which is absorbed at nearly 100% efficiency since it is delivered directly into the bloodstream
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[CrossRef] [PubMed] [Google Scholar] Vitamin B(12) deficiency and depression in physically disabled older women: Epidemiologic evidence from the Women's Health and Aging Study
Some people also find that pressing firmly near (but not at) the injection site with a finger for a few seconds before inserting the needle provides competing sensory input that reduces pain perception
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